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| Zendong, Suzie Zita; Kadiri, Medina; Herrenknecht, Christine; Nezan, Elisabeth; Mazzeo, Antonia; Hess, Philipp. |
| Algal toxins may accumulate in fish and shellfish and thus cause poisoning in consumers of seafood. Such toxins and the algae producing them are regularly surveyed in many countries, including Europe, North America, Japan and others. However, very little is known regards the occurrence of such algae and their toxins in most African countries. This paper reports on a survey of phytoplankton and algal toxins in Nigerian coastal waters. Seawater samples were obtained from four sites for phytoplankton identification, on three occasions between the middle of October 2014 and the end of February 2015 (Bar Beach and Lekki in Lagos State, Port Harcourt in Rivers State and Uyo in Akwa Ibom State). The phytoplankton community was generally dominated by diatoms and... |
| Tipo: Text |
Palavras-chave: Dinoflagellates; Dinophysis; Phycotoxins; Untargeted analysis; Phytoplankton. |
| Ano: 2016 |
URL: http://archimer.ifremer.fr/doc/00314/42521/41893.pdf |
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| Zendong, Suzie Zita; Mccarron, Pearse; Herrenknecht, Christine; Sibat, Manoella; Amzil, Zouher; Cole, Richard B.; Hess, Philipp. |
| Measurement of marine algal toxins has traditionally focussed on shellfish monitoring while, over the last decade, passive sampling has been introduced as a complementary tool for exploratory studies. Since 2011, liquid chromatography-tandem mass spectrometry (LC-MS/MS) has been adopted as the EU reference method (No.15/2011) for detection and quantitation of lipophilic toxins. Traditional LC-MS approaches have been based on low-resolution mass spectrometry (LRMS), however, advances in instrument platforms have led to a heightened interest in the use of high-resolution mass spectrometry (HRMS) for toxin detection. This work describes the use of HRMS in combination with passive sampling as a progressive approach to marine algal toxin surveys. Experiments... |
| Tipo: Text |
Palavras-chave: Monitoring; Marine toxins; Passive sampling; SPATT; Matrix effects. |
| Ano: 2015 |
URL: http://archimer.ifremer.fr/doc/00277/38848/37388.pdf |
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| Hess, Philipp; Mondeguer, Florence; Glauner, Thomas; Wust, Bernard; Sibat, Manoella; Zendong, Suzie Zita; Herrenknecht, Christine; Sechet, Veronique. |
| Out of the circa 5000 microalgae known, around 100 marine species of unicellular algae have been shown to produce several hundred biotoxins worldwide. Such biotoxins may be harmful to man through consumption of fish and shellfish exposed to such algae or through direct contact. Many official methods aimed at the detection of such toxins had traditionally been based on biological assays, due to the lack of reference compounds. Recently, legislation for shellfish safety in Europe introduced targeted MS screening for lipophilic toxins. Due to globalization of trade and climate change, there is a need to develop methods for untargeted screening to fully protect consumer safety, e.g. LC-HRMS. For this purpose we studied the metabolomes of marine microalgae... |
| Tipo: Text |
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| Ano: 2013 |
URL: http://archimer.ifremer.fr/doc/00179/29037/27473.pdf |
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| Zendong, Suzie Zita; Herrenknecht, Christine; Mondeguer, Florence; Hess, Philipp. |
| By 2014, the new targeted LC-MS/MS reference method for the detection of lipophilic toxins will replace the mouse bioassay (MBA). This bioassay, which has the advantage of being a rapid and global toxicity test, can not be used for toxin identification purposes. Furthermore, it has appeared that, in some cases, the mouse bioassay could reveal uncharacterized toxicities that could not be elucidated by targeted mass spectrometry methods. Therefore, moving from the global toxicity assessment test to a targeted technique leads to a lack of information on emerging toxins. Objectives: - Beside targeted methods, develop metabolomic approaches to screen for known and unknown emerging toxins - Develop passive sampling devices suitable for toxins of varying... |
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| Ano: 2013 |
URL: http://archimer.ifremer.fr/doc/00179/29040/27478.pdf |
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| Mondeguer, Florence; Abadie, Eric; Herve, Fabienne; Bardouil, Michele; Sechet, Veronique; Raimbault, Virginie; Berteaux, Tom; Zendong, Suzie Zita; Palvadeau, Hubert; Amzil, Zouher; Hess, Philipp; Fessard, Valérie; Huguet, Antoine; Sosa, Silvio; Tubaro, Aurelia; Aráoz, Rómulo; Molgó, Jordi. |
| Pour tenter d’apporter des réponses aux questions soulevées par une précédente étude commanditée pour approfondir les connaissances sur Vulcanodinium rugosum, l’organisme producteur de la Pinnatoxine G (PnTX G) (Hess et al., 2012), les objectifs de cette nouvelle étude sont les suivants : - Elargissement de la zone initiale de prélèvement d’Ingril à toute la lagune méditerranéenne et mise à profit de l’augmentation du nombre d’échantillons pour vérifier l’impact de l’effet matrice dans l’analyse LC-SM/MS. - Mise en culture en masse de Vulcanodinium rugosum et - Conduite d’une expérience de contamination in vivo sur des moules pour vérifier l’accumulation de la PnTX-G et comprendre les mécanismes de la métabolisation des PnTXs et de ses composés associés..... |
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| Ano: 2015 |
URL: http://archimer.ifremer.fr/doc/00285/39635/38126.pdf |
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| Zendong, Suzie Zita; Sibat, Manoella; Herrenknecht, Christine; Hess, Philipp; Mccarron, Pearse. |
| Rationale Accurate quantitative analysis of lipophilic toxins by liquid chromatography-mass spectrometry (LC-MS) requires calibration solution reference materials (RMs) for individual toxin analogs. Untargeted analysis is aimed at identifying a vast number of compounds and thus validation of fully quantitative untargeted methods is not feasible. However, a semi-quantitative approach allowing for profiling is still required and will be strengthened by knowledge of the relative molar response (RMR) of analogs in liquid chromatography-mass spectrometry (LC-MS) with electrospray ionization (ESI). Methods RMR factors were evaluated for toxins from the okadaic acid (OA/DTXs), yessotoxin (YTX), pectenotoxin (PTX), azaspiracid (AZA) and cyclic imine (CI) toxin... |
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| Ano: 2017 |
URL: http://archimer.ifremer.fr/doc/00387/49877/50434.pdf |
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